Cdk2 Antibody Cell Signaling

To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.

Cdk2 antibody cell signaling.

Phospho cdk2 thr160 antibody detects endogenous levels of cdk2 only when phosphorylated at threonine 160. Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells. It does not cross react with other phosphorylated cyclin dependent kinases. The antibody weakly cross reacts with cdc2 phosphorylated at thr161.

Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100. Kinase activity associated with cyclin a cdc2 is found only in g2 phase. Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412. Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.

An antibody shouldn t be one of the variables in your experiment. Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen. Merged red and green signal. Inhibitory phosphorylation occurs on thr14 and tyr15 1.

Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2. Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.

Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Antibodies are purified by protein a and peptide affinity chromatography.

Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor. Inhibitory phosphorylation occurs on thr14 and tyr15 1. Mix well then add 0 1 g bsa mix. Wild type hap1 cell lysate 20 µg lanes 2 4 and 6.

Find out why customers rank cst highest for antibody specificity and sensitivity. Recommended fluorochrome conjugated anti rabbit secondary antibodies. Green signal from target ab32147 observed at 34 kda lanes 3 and 4. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.

Western blot analysis was performed using cdk12 antibody. Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.